Infrared Based Saliva Screening Test for COVID-19

BR Wood; K Kochan; DE Bedolla; N Salazar-Quiroz; SL Grimley; D Perez-Guaita; MJ Baker; J Vongsvivut; MJ Tobin; KR Bambery; D Christensen; S Pasricha; AK Eden; A Mclean; S Roy; JA Roberts; J Druce; DA Williamson; J McAuley; M Catton; DFJ Purcell; DI Godfrey; P Heraud

Journal article

Infrared Based Saliva Screening Test for COVID-19

BR Wood, K Kochan, DE Bedolla, N Salazar-Quiroz, SL Grimley, D Perez-Guaita, MJ Baker, J Vongsvivut, MJ Tobin, KR Bambery, D Christensen, S Pasricha, AK Eden, A Mclean, S Roy, JA Roberts, J Druce, DA Williamson, J McAuley, M Catton Show all

Angewandte Chemie International Edition | Published : 2021

DOI: 10.1002/anie.202104453

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sen..

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University of Melbourne Researchers

Samantha Grimley Author

Shivani Pasricha Author

Jason Roberts Author

Julian Druce Author

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Grants

Awarded by Australian Nuclear Science and Technology Organisation

Funding Acknowledgements

Mr. Finlay Shanks for instrumental support. Dr Emanuele Pedersoli for his assistance in graphic scripting. Funding: Partof this research was undertaken on the IRM beamline at Australian Synchrotron (Victoria, Australia), part of the Australian Nuclear Science and Technology Organisation (ANSTO). We acknowledge the support of the beamtime (Proposal ID. 16460 and 16476) provided by ANSTO, funded by the Australian Government. This work was in-part funded by Monash Green Chemical Futures Special Project fund. Part of this work was funded by the A2 milk foundation. Instruments were loaned and modified for transflection infrared slides by Dr Jerry Sellors and Dr Robert Packer courtesy of PerkinElmerU. N.S.Q, S.G. and J.M. were supported by funds from the Jack Ma Foundation through a grant to D.P. and D.I.G. D.P. and D.I.G. were supported by the National Health and Medical Research Council of Australia (NHMRC), including a Senior Principal Research Fellowship (1117766) and the Australian Research Council CE140100011 for D.I.G. D.P.-G. acknowledges the financial support from the 2019 Ramln y Cajal (RYC) Contracts Aids (RYC2019026556-I).